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sample analysis
DDsite and DDsite-Cyto

Tag. Retrieve. Discover.

Designed for use with cells exposed to DNA-damaging treatments, DDsite delivers unparalleled insight into genomic instability and its implications for drug efficacy and precision medicine.

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Get a complimentary
sample analysis

How it works

Locate frequent and persistent DNA break sites across the genome

Step 1
Culture your cells and treat with your drug or treatment of interest to induce DNA damage.
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Step 2
Freeze down the cells, package, and ship the samples to us.
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Step 3
At BreakSight, we’ll tag DNA double-strand break ends within the nucleus or cytoplasm of your cells.
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Step 4
We’ll extract and shear genomic DNA before specifically retrieving the labeled DNA break fragments.
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Step 5
Samples containing the retrieved DNA break fragments and the genomic input will then undergo next-generation sequencing (NGS).
Includes:​

Sample QC
Sample fragmentation
Library Prep
Library QC

150 bp paired-end sequencing (~60 million read pairs per sample)

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Results

Example of BrITL qRT-PCR results

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DDinsight and DDinsight+

Gain insights into your data

DDinsight processes and analyzes sequenced data to identify reproducible DNA break sites, compare treatment effects, and pinpoint enriched DNA motifs or repeats.

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DDsite
Discover a deeper understanding of genomic instability
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